WorkBeads™ Macro SEC resin is used for preparative size exclusion chromatography (SEC) in laboratory and process scale purification of proteins, virus and other biomolecules by utilizing differences in their size. This resin is based on agarose, a biopolymer suitable for separation of biomolecules.
WorkBeads resins are cross-linked using a proprietary method that results in a very rigid, highly porous and physically stable matrix.
Although the general recommendation for SEC is to use low flow rate for best purification, the rigidity and tight particle size distribution allows purification of viruses and other large substances at high flow rate for fast processing and high yields.
- Alternative bead sizes for viscous samples
- Resistant to harsh cleaning agents (NaOH)
- Rigidity and tight particle size distribution allows purification of large substances at high flow rate for fast processing and high yields.
| WorkBeads Macro SEC |
| Matrix | Rigid, highly cross-linked agarose |
Separation range¹ | 10 - 30 000 kD |
Exclusion limit | 30 000 kD |
Average particle size² (Dv50) | 45 µmol |
Recommended flow rate³ | 15 - 150 cm/h |
Max flow rate⁴˒⁵ | 300 cm/h |
Chemical stability | Compatible with all standard aqueous buffers used for protein purification. Should not be stored at low pH for prolonged time. |
pH stability | 2 – 13 |
Storage | 2 to 25 °C in 20% ethanol |