BabyBio™ NTA columns for Immobilized Metal Ion Affinity Chromatography (IMAC) are ready-to-use for quick and easy purification of polyhistidine-tagged (His-tagged) proteins and other proteins with an affinity for metal ions. The columns are prepacked with pre-charged or uncharged WorkBeads™ 40 NTA resins. They are available in two column sizes, 1 ml and 5 ml. IMAC utilizes the affinity of histidine, cysteine and tryptophan amino acid side chains on the protein surface for binding to transition metal ions, such as Ni2+, Co2+, Cu2+ and Zn2+, which are immobilized via the metal chelating ligand on the chromatography resin.
- High binding capacity and purity
- Pre-charged resins with different metal ions for convenient screening for optimal purity
- Uncharged resins can be charged wih the metal ion of choice
- Prepacked ready-to-use columns for rapid and reliable results
