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Picture of VP 125/10 NUCLEOGEN 60-7 DEAE 736597.100

VP 125/10 NUCLEOGEN 60-7 DEAE 736597.100

HPLC column for special application VP 125/10 NUCLEOGEN 60-7 DEAE
SKU: 736597.100
Manufacturer: Machery Nagel

 

Silica-based HPLC phase for anion exchange chromatography of nucleic acids. For separation of oligonucleotides up to chain lengths of 40 bases with recoveries > 95 %. Capacity 200 A260/mL (~ 300 A260 for a 125 x 4 mm ID column, 1875 A260 for a 125 x 10 mm ID column). Preparative separations possible when using higher flow rates and longer gradient times.

Hazardous materialNo
MethodHPLC, preparative
BrandNUCLEOGEN
Base materialSilica gel
PhaseNUCLEOGEN 60-7 DEAE
USP listingNot available or proprietary
Surface chemistrySilica gel based anion exchanger with hydrophilic coating
ModeIon exchange (IEX), Special application or diverse
TypeVP HPLC column (preparative)
HardwareStainless steel
Column length125 mm
Column inner diameter10 mm
Particle typeFully porous particles (FPP)
Particle shapeSpherical
Particle size7 µm
Eluent in HPLC columnMethanol
Recommended application(s)HPLC, Oligonucleotides up to chain lengths of 40 bases
EndcappedNo
Carbon contentNot available or proprietary
pH stability2.5–8.0
Pore size60 Å
Specific surface according to BETNot available or proprietary
Pore volumeNot available or proprietary
Temperature stability (max)70 °C
Pressure stability (max)250 bar
Storage temperatureRT
Scope of delivery1x semi-preparative HPLC column, instruction leaflet, certificate of analysis

 

Attribute nameAttribute value
MethodHPLC, preparative
BrandNUCLEOGEN
Phase60-7 DEAE
Column Size125x10mm
Particle size7 µm

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